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大鼠高敏型心肌肌鈣蛋白T(hs-cTnT)酶聯(lián)免疫吸附檢測試劑盒

一鍵復制產(chǎn)品信息

ELK0844

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00
96T*5	￥13600.00

下載說明 ①

Overview 文獻(2)

Product name:	Rat hs-cTnT(High Sensitivity Cardiac Troponin T) ELISA Kit
Reactivity:	Rat
Alternative Names:	High Sensitivity Cardiac Troponin T
Assay Type:	Sandwich
Sensitivity:	5.2 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cardiovascular biology
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-cTnT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-cTnT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-cTnT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hs-cTnT in the samples is then determined by comparing the OD of the samples to the standard curve.

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標準曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.075	1.992
500.00	1.630	1.547
250.00	1.116	1.033
125.00	0.815	0.732
62.50	0.539	0.456
31.25	0.313	0.230
15.63	0.252	0.169
0.00	0.083	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant hs-cTnT and the recovery rates were calculated by comparing the measured value to the expected amount of hs-cTnT in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	79-93%	86%
Heparin plasma(n=5)	82-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-cTnT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.