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一步法人血管緊張素Ⅱ(AngII)酶聯(lián)免疫吸附檢測(cè)試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK098ES

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00

下載說(shuō)明 ①

Overview

Product name:	EasyStep Human AngII(Angiotensin II) ELISA Kit
Reactivity:	Human
Alternative Names:	Ang-II; Angiotensin-2
Assay Type:	Competitive Inhibition
Sensitivity:	10.63 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Endocrinology;Cardiovascular biology;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AngII antigen, and the Human AngII standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human AngII to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human AngII in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	0.197
500.00	0.396
250.00	0.527
125.00	0.857
62.50	1.169
31.25	1.457
15.63	1.796
0.00	2.112

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant AngII and the recovery rates were calculated by comparing the measured value to the expected amount of AngII in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	81-95%	88%
Heparin plasma(n=5)	90-105%	97%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AngII and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.