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小鼠高遷移率族蛋白B1(HMGB-1)酶聯(lián)免疫吸附檢測(cè)試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK10273

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻(xiàn)(2)

Product name:	Mouse HMGB-1(High Mobility Group Protein B1) ELISA Kit
Reactivity:	Mouse
Alternative Names:	HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; HMG1; High Mobility Group Protein 1
Assay Type:	Sandwich
Sensitivity:	0.066 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample type:	serum, plasma and other biological fluids
Assay length:	3.5h
Research Area:	Infection immunity;
Uniprot ID:	P63158
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Journal of Advanced Research

Deficiency of myeloid NPC1 exacerbates liver injury and fibrosis by impairing macrophage efferocytosis

日期：2025-01-17 IF：11.4
Frontiers in Molecular Neuroscience

Netrin-1 Ameliorates Postoperative Delirium-Like Behavior in Aged Mice by Suppressing Neuroinflammation and Restoring Impaired Blood-Brain Barrier Permeability

日期：2024-07-17 IF：6.2

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.266	2.198
5.00	1.593	1.525
2.50	1.183	1.115
1.25	0.752	0.684
0.63	0.553	0.485
0.32	0.325	0.257
0.16	0.225	0.157
0.00	0.068	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant HMGB-1 and the recovery rates were calculated by comparing the measured value to the expected amount of HMGB-1 in samples.

Matrix	Recovery range	Average
serum(n=5)	79-97%	88%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	84-98%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HMGB-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.