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8-羥基脫氧鳥苷(8-OHdG)酶聯(lián)免疫吸附檢測試劑盒
一鍵復(fù)制產(chǎn)品信息
ELK10848
規(guī)格: 價(jià)格:
48T ¥1680.00
96T ¥2400.00

Overview 文獻(xiàn)(5)

Product name: 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
Reactivity: General
Alternative Names: 8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 7,8-dihydrodeoxyguanosine
Assay Type: Competitive Inhibition
Sensitivity: 0.57 ng/mL
Standard: 100 ng/mL
Detection Range: 1.56-100 ng/mL
Sample type: serum, plasma and other biological fluids
Assay length: 2h
Research Area: Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-OHdG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL) OD Corrected OD
100.00 0.221
50.00 0.411
25.00 0.692
12.50 0.913
6.25 1.225
3.13 1.587
1.57 1.813
0.00 2.325

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 8-OHdG and the recovery rates were calculated by comparing the measured value to the expected amount of 8-OHdG in samples.
Matrix Recovery range Average
serum(n=5) 94-107% 100%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 94-105% 87%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-OHdG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-105% 92-101% 80-93% 89-97%
EDTA plasma(n=5) 85-90% 83-96% 95-102% 88-95%
Heparin plasma(n=5) 92-102% 83-89% 88-104% 97-103%
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