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大鼠游離睪酮(F-Testo)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK10934

規(guī)格：	價(jià)格：
48T	￥1680.00
96T	￥2400.00
96T*5	￥10200.00

下載說明 ①

Overview 文獻(xiàn)(2)

Product name:	Rat F-Testo(Free Testosterone) ELISA Kit
Reactivity:	Rat
Alternative Names:	F-TESTO; Free Testosterone
Assay Type:	Competitive Inhibition
Sensitivity:	16.3 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	serum, plasma, other biological fluids
Assay Length:	2h
Research Area:	Endocrinology;Reproductive science;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat F-Testo. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F-Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F-Testo in the samples is then determined by comparing the OD of the samples to the standard curve.

Pharmaceutical Biology

Baicalin suppress the development of polycystic ovary syndrome via regulating the miR-874-3p/FOXO3 and miR-144/FOXO1 axis

日期：2023-04-24 IF：3.889
BMC Complementary Medicine and Therapies

Probiotics mitigates ovarian angiogenic disturbance in letrozole-induced PCOS

日期：2025-11-03 IF：3.4

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
2000.00	0.227
1000.00	0.332
500.00	0.621
250.00	0.846
125.00	1.139
62.50	1.565
31.25	1.755
0.00	2.325

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant F-Testo and the recovery rates were calculated by comparing the measured value to the expected amount of F-Testo in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	86-99%	92%
Heparin plasma(n=5)	82-95%	98%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F-Testo and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.