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人白介素12 p40(IL-12p40)微量上樣酶聯(lián)免疫吸附檢測試劑盒

一鍵復制產品信息

ELK2263MS

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview

Product name:	Human IL-12p40(Interleukin 12 p40) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	IL-12B; p40; IL12B; CLMF2; NKSF2; Natural Killer Cell Stimulatory Factor 2; Cytotoxic Lymphocyte Maturation Factor 2; Interleukin 23, P40 Subunit; Interleukin 12B
Assay Type:	Sandwich
Sensitivity:	2.9 pg/mL
Standard:	500 pg/mL
Detection Range:	7.82-500 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length:	3.5h
Research Area:	Cytokine;Infection immunity;Immune molecule;
Uniprot ID:	P29460
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-12p40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-12p40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-12p40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-12p40 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
500.00	2.067	1.968
250.00	1.661	1.562
125.00	1.244	1.145
62.50	0.909	0.810
31.25	0.525	0.426
15.63	0.321	0.222
7.81	0.173	0.074
0.00	0.099	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL-12p40 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12p40 in samples.

Matrix	Recovery range	Average
serum(n=5)	91-111%	101%
EDTA plasma(n=5)	84-105%	95%
Heparin plasma(n=5)	89-114%	102%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12p40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.