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大鼠巨噬細胞炎性蛋白1β(MIP1b)微量上樣酶聯(lián)免疫吸附檢測試劑盒

一鍵復制產(chǎn)品信息

ELK2545MS

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview

Product name:	Rat MIP1b(Macrophage Inflammatory Protein 1 Beta) Microsample ELISA Kit
Reactivity:	Rat
Alternative Names:	MIP1 Beta; CCL4; ACT2; G-26; LAG1; MIP1-B; SCYA4; Chemokine C-C-Motif Ligand 4; Small Inducible Cytokine A4; Homologous To Mouse Mip-1b; CCL4L1; C C motif chemokine ligand 4 like 1; CC chemokine ligand 4L1; C-C motif chemokine 4-like; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; Monocyte adherence-induced protein 5-alpha; Small-inducible cytokine A4-like; CCL4L; SCYA4L1; CCL4L2; SCYA4L2
Assay Type:	Sandwich
Sensitivity:	31 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Length:	3.5h
Research Area:	Cytokine;Infection immunity;
Uniprot ID:	P50230
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MIP1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MIP1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MIP1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MIP1b in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.146	2.059
2500.00	1.437	1.350
1250.00	0.937	0.850
625.00	0.755	0.668
312.50	0.506	0.419
156.25	0.373	0.286
78.13	0.200	0.113
0.00	0.087	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant MIP1b and the recovery rates were calculated by comparing the measured value to the expected amount of MIP1b in samples.

Matrix	Recovery range	Average
serum(n=5)	93-118%	106%
EDTA plasma(n=5)	93-114%	104%
Heparin plasma(n=5)	90-106%	98%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIP1b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.