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小鼠可溶性白介素6受體(sIL-6R)微量上樣酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK3775MS

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview

Product name:	Mouse sIL-6R(SolubleInterleukin 6 Receptor) Microsample ELISA Kit
Reactivity:	Mouse
Alternative Names:	IL-6R;CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80; IL6R; Interleukin 6 Receptor
Assay Type:	Sandwich
Sensitivity:	0.118 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample Type:	serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;CD & Adhesion molecule;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse sIL-6R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse sIL-6R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse sIL-6R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse sIL-6R in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
20.00	2.194	2.106
10.00	1.579	1.491
5.00	1.224	1.136
2.50	0.908	0.820
1.25	0.526	0.438
0.63	0.372	0.284
0.31	0.220	0.132
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant sIL-6R and the recovery rates were calculated by comparing the measured value to the expected amount of sIL-6R in samples.

Matrix	Recovery range	Average
serum(n=5)	89-116%	103%
EDTA plasma(n=5)	83-125%	104%
Heparin plasma(n=5)	81-100%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sIL-6R and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.