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大鼠生長分化因子9(GDF9)微量上樣酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK5051MS

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview

Product name:	Rat GDF9(Growth Differentiation Factor 9) Microsample ELISA Kit
Reactivity:	Rat
Alternative Names:	GDF9Growth/differentiation factor 9 ; GDF-9
Assay Type:	Sandwich
Sensitivity:	28 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids.
Assay Length:	3.5h
Research Area:	Cytokine;Metabolic pathway;Developmental science;Bone metabolism;
Uniprot ID:	Q9Z0X3
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GDF9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GDF9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GDF9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GDF9 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
5000.00	1.704	1.614
2500.00	1.406	1.316
1250.00	1.203	1.113
625.00	0.962	0.872
312.50	0.601	0.511
156.25	0.347	0.257
78.13	0.174	0.084
0.00	0.090	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant GDF9 and the recovery rates were calculated by comparing the measured value to the expected amount of GDF9 in samples.

Matrix	Recovery range	Average
serum(n=5)	81-92%	87%
EDTA plasma(n=5)	81-115%	98%
Heparin plasma(n=5)	83-114%	99%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GDF9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.