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人唾液酸結合Ig樣凝集素7(SIGLEC7)微量上樣酶聯免疫吸附檢測試劑盒

一鍵復制產品信息

ELK5193MS

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview

Product name:	Human SIGLEC7(Sialic Acid Binding Ig Like Lectin 7) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	CDw328; CD328; p75; AIRM1; D-Siglec; QA79; SIGLEC-7; P75/AIRM1; Adhesion inhibitory receptor molecule 1; QA79 membrane protein
Assay Type:	Sandwich
Sensitivity:	0.059 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids.
Assay Length:	3.5h
Research Area:	CD & Adhesion molecule;Tumor immunity;Infection immunity;
Uniprot ID:	Q9Y286
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SIGLEC7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SIGLEC7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SIGLEC7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SIGLEC7 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.069	1.984
5.00	1.596	1.511
2.50	1.114	1.029
1.25	0.768	0.683
0.63	0.606	0.521
0.31	0.242	0.157
0.16	0.207	0.122
0.00	0.085	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant SIGLEC7 and the recovery rates were calculated by comparing the measured value to the expected amount of SIGLEC7 in samples.

Matrix	Recovery range	Average
serum(n=5)	92-111%	102%
EDTA plasma(n=5)	90-128%	109%
Heparin plasma(n=5)	84-114%	99%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIGLEC7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.