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人B-淋巴細(xì)胞激活抗原B7-1(LAB7-1)微量上樣酶聯(lián)免疫吸附檢測(cè)試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK5326MS

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview

Product name:	Human LAB7-1(B-Lymphocyte Activation Antigen B7-1) Microsample ELISA Kit
Reactivity:	Human
Alternative Names:	CD80; CD28LG; CD28LG1; BB1; B7-1 Antigen; T-lymphocyte activation antigen CD80; CTLA-4 counter-receptor B7.1
Assay Type:	Sandwich
Sensitivity:	32 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids
Assay Length:	3.5h
Research Area:	CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;Autoimmunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAB7-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAB7-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAB7-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAB7-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.249	2.165
2500.00	1.800	1.716
1250.00	1.247	1.163
625.00	0.856	0.772
312.50	0.472	0.388
156.25	0.301	0.217
78.13	0.229	0.145
0.00	0.084	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant LAB7-1 and the recovery rates were calculated by comparing the measured value to the expected amount of LAB7-1 in samples.

Matrix	Recovery range	Average
serum(n=5)	94-120%	107%
EDTA plasma(n=5)	90-106%	98%
Heparin plasma(n=5)	86-119%	103%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LAB7-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.