,

產(chǎn)品中心

大鼠白介素2受體a鏈(IL-2Ra/CD25)微量上樣酶聯(lián)免疫吸附檢測(cè)試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK5694MS

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00
96T*5	￥13600.00

下載說明 ①

Overview

Product name:	Rat IL-2sRa/CD25(Soluble Interleukin-2 Receptor alpha chain) Microsample ELISA Kit
Reactivity:	Rat
Alternative Names:	IL-2RA;CD25; IL2R-a; IL2-RA; IDDM10; IL2R; TCGFR; p55; TAC antigen; IL2Ra; Interleukin 2 Receptor Alpha
Assay Type:	Sandwich
Sensitivity:	0.06 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;CD & Adhesion molecule;Tumor immunity;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL-2sRa/CD25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL-2sRa/CD25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL-2sRa/CD25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.399	2.304
5.00	1.665	1.570
2.50	1.197	1.102
1.25	0.937	0.842
0.63	0.527	0.432
0.31	0.368	0.273
0.16	0.200	0.105
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL-2sRa/CD25 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-2sRa/CD25 in samples.

Matrix	Recovery range	Average
serum(n=5)	91-118%	105%
EDTA plasma(n=5)	87-107%	97%
Heparin plasma(n=5)	83-88%	86%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-2sRa/CD25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.