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人血管緊張素1-7(Ang1-7)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK7678

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview

Product name:	Human Ang1-7(Angiotensin 1-7) ELISA Kit
Reactivity:	Human
Alternative Names:	Ang1-7
Assay Type:	Sandwich
Sensitivity:	4.62 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	3.5h
Research Area:	Endocrinology;Cardiovascular biology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ang1-7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ang1-7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ang1-7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ang1-7 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	1.946	1.864
500.00	1.587	1.505
250.00	1.157	1.075
125.00	0.897	0.815
62.50	0.508	0.426
31.25	0.385	0.303
15.63	0.226	0.144
0.00	0.082	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Ang1-7 and the recovery rates were calculated by comparing the measured value to the expected amount of Ang1-7 in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	88-102%	95%
Heparin plasma(n=5)	95-107%	101%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ang1-7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.