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人抗白蛋白抗體(Anti-ALB)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK7831

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00
96T*5	￥13600.00

下載說明 ①

Overview 文獻(xiàn)(1)

Product name:	Human Anti-ALB(Anti-Albumin Antibody) ELISA Kit
Reactivity:	Human
Alternative Names:	Serum albumin Ab
Assay Type:	Competitive Inhibition
Sensitivity:	1.3 ng/mL
Standard:	200 ng/mL
Detection Range:	3.13-200 ng/mL
Sample Type:	Serum, plasma and other biological fluids
Assay Length:	2.5h
Research Area:	Tumor immunity;Infection immunity;Hematology;Hepatology;Nutrition metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-ALB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-ALB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-ALB in the samples is then determined by comparing the OD of the samples to the standard curve.

AMB Express

SETDB1 knockdown boosts recombinant protein in CHO cells via epigenetic transcriptional silencing

日期：2025-08-15 IF：3.7

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
200.00	0.197
100.00	0.357
50.00	0.596
25.00	0.812
12.50	1.197
6.25	1.583
3.13	1.796
0.00	2.112

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Anti-ALB and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-ALB in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	80-92%	86%
Heparin plasma(n=5)	85-97%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-ALB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.