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大鼠線粒體開放閱讀框12S rRNA-C(MOTS-c)酶聯免疫吸附檢測試劑盒

一鍵復制產品信息

ELK8233

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00
96T*5	￥13600.00

下載說明 ①

Overview 文獻(1)

Product name:	Rat MOTS-c(Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit
Reactivity:	Rat
Alternative Names:	MOTS-c
Assay Type:	Competitive Inhibition
Sensitivity:	0.97 ng/mL
Standard:	200 ng/mL
Detection Range:	3.13-200 ng/mL
Sample Type:	serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length:	2h
Research Area:	Developmental science;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MOTS-c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MOTS-c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MOTS-c in the samples is then determined by comparing the OD of the samples to the standard curve.

Molecular Neurobiology

The Mitochondrial-Derived Peptide (MOTS-c) Interacted with Nrf2 to Defend the Antioxidant System to Protect Dopaminergic Neurons Against Rotenone Exposure

日期：2023-06-10 IF：5.1

標準曲線

Concentration (ng/mL)	OD	Corrected OD
200.00	0.185
100.00	0.413
50.00	0.596
25.00	0.997
12.50	1.197
6.25	1.522
3.13	1.889
0.00	2.101

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant MOTS-c and the recovery rates were calculated by comparing the measured value to the expected amount of MOTS-c in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	78-90%	84%
Heparin plasma(n=5)	89-107%	90%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MOTS-c and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.