国产亚洲精品精品国产亚洲综合|亚洲无码在线免费视频|久久精品国产久精国产思思 ,能在手机看的不卡av,操逼逼网,人人澡人人干,久草XX ,无码一区二区在线欧洲_亚洲欧美日韩成人一区久久

產(chǎn)品中心

小鼠線粒體超氧化物歧化酶(SOD2)微量上樣酶聯(lián)免疫吸附檢測(cè)試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK8590MS

規(guī)格：	價(jià)格：
48T	￥2240.00
96T	￥3200.00
96T*5	￥13600.00

下載說(shuō)明 ①

Overview

Product name:	Mouse SOD2(Superoxide Dismutase 2, Mitochondrial) Microsample ELISA Kit
Reactivity:	Mouse
Alternative Names:	IPO-B; MNSOD; Mn-SOD
Assay Type:	Sandwich
Sensitivity:	13.5 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Enzyme & Kinase;Metabolic pathway;Tumor immunity;Cardiovascular biology;Neuro science;
Uniprot ID:	P09671
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SOD2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SOD2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SOD2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SOD2 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (pg/mL)	OD	Corrected OD
2000.00	1.959	1.868
1000.00	1.597	1.506
500.00	1.158	1.067
250.00	0.808	0.717
125.00	0.503	0.412
62.50	0.382	0.291
31.25	0.211	0.120
0.00	0.091	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant SOD2 and the recovery rates were calculated by comparing the measured value to the expected amount of SOD2 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-118%	101%
EDTA plasma(n=5)	87-123%	105%
Heparin plasma(n=5)	92-99%	96%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SOD2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.