久久久久精品一级毛片_中日无码电影在线_国产情侣久久精品_国自产拍91中文在线观看_无码人妻精品 ,久久亚洲国产成人精品无码区_zσzσzσ女人极品另类_久久性爱视频_色橹橹欧美在线观看视频高清 ,九一香蕉视频_香蕉视频网站_色婷婷香蕉在线一区二区

產(chǎn)品中心

大鼠抗增殖細(xì)胞核抗原(PCNA)微量上樣酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK8614MS

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Rat PCNA(Proliferating Cell Nuclear Antigen) Microsample ELISA Kit
Reactivity:	Rat
Alternative Names:	Cyclin
Assay Type:	Sandwich
Sensitivity:	0.054 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Tumor immunity;Developmental science;
Uniprot ID:	P04961
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PCNA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PCNA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PCNA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PCNA in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	2.137	2.043
5.00	1.783	1.689
2.50	1.129	1.035
1.25	0.871	0.777
0.63	0.505	0.411
0.31	0.304	0.210
0.16	0.217	0.123
0.00	0.094	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PCNA and the recovery rates were calculated by comparing the measured value to the expected amount of PCNA in samples.

Matrix	Recovery range	Average
serum(n=5)	89-97%	93%
EDTA plasma(n=5)	83-127%	105%
Heparin plasma(n=5)	84-97%	91%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PCNA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.