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人鳳凰蛋白20(PNX-20)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK8899

規(guī)格：	價格：
48T	￥2240.00
96T	￥3200.00

下載說明 ①

Overview

Product name:	Human PNX-20(Phoenixin-20) ELISA Kit
Reactivity:	Human
Alternative Names:	Small integral membrane protein; Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 7 kDa; MITRAC7; Phoenixin-14; PNX-14; Phoenixin-20; PNX-20; SMIM20; C4orf52
Assay Type:	Sandwich
Sensitivity:	7.3 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample type:	Serum, plasma and other biological fluids.
Assay length:	3.5h
Research Area:	Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Uniprot ID:	Q8N5G0
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PNX-20. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PNX-20. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PNX-20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PNX-20 in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.266	2.171
500.00	1.642	1.547
250.00	1.196	1.101
125.00	0.821	0.726
62.50	0.585	0.490
31.25	0.325	0.230
15.63	0.241	0.146
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant PNX-20 and the recovery rates were calculated by comparing the measured value to the expected amount of PNX-20 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	85-98%	91%
Heparin plasma(n=5)	96-107%	102%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PNX-20 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.