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人抗心磷脂抗體IgG(ACA-IgG)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK9253

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit
Reactivity:	Human
Alternative Names:	ACA-IgG; Anti-Cardiolipin Antibody IgG
Assay Type:	Sandwich
Sensitivity:	1.076 ng/mL
Standard:	200 ng/mL
Detection Range:	3.13-200 ng/mL
Sample type:	Serum, plasma, tissue homogenates and other biological fluids
Assay length:	3.5h
Research Area:	Cardiovascular biology
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACA-IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACA-IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACA-IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACA-IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
200.00	1.929	1.843
100.00	1.543	1.457
50.00	1.115	1.029
25.00	0.821	0.735
12.50	0.557	0.471
6.25	0.319	0.233
3.13	0.206	0.120
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant ACA-IgG and the recovery rates were calculated by comparing the measured value to the expected amount of ACA-IgG in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	87-101%	94%
Heparin plasma(n=5)	89-103%	96%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACA-IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.