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人抗心磷脂抗體IgM(ACA-IgM)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK9254

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Human ACA-IgM(Anti-Cardiolipin Antibody IgM) ELISA Kit
Reactivity:	Human
Alternative Names:	ACA-IgM; Anti-Cardiolipin Antibody IgM
Assay Type:	Sandwich
Sensitivity:	0.081 ng/mL
Standard:	20 ng/mL
Detection Range:	0.31-20 ng/mL
Sample type:	serum, plasma and other biological fluids
Assay length:	3.5h
Research Area:	Cardiovascular biology
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACA-IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACA-IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACA-IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACA-IgM in the samples is then determined by comparing the OD of the samples to the standard curve.

標準曲線

Concentration (ng/mL)	OD	Corrected OD
20.00	2.258	2.157
10.00	1.673	1.572
5.00	1.182	1.081
2.50	0.928	0.827
1.25	0.655	0.554
0.63	0.369	0.268
0.32	0.252	0.151
0.00	0.101	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant ACA-IgM and the recovery rates were calculated by comparing the measured value to the expected amount of ACA-IgM in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	87-95%	91%
Heparin plasma(n=5)	80-97%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACA-IgM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.