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斑馬魚熱休克蛋白70(HSP-70/HSPA9)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK9341

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview 文獻(1)

Product name:	Zebrafish HSP-70/HSPA9(HeatShock Protein 70) ELISA Kit
Reactivity:	Zebrafish
Alternative Names:	DnaK; 70 Kilodalton Heat Shock Proteins;Heat shock 70 kDa protein 1; 2; heat shock 70kDa protein 1A; HSP70; HSP70 1; HSP70 1; HSP70 2; HSP70 1A; HSP70.1; HSP70.2; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B; HSP70; Heat Shock Protein 70
Assay Type:	Sandwich
Sensitivity:	1.33 ng/mL
Standard:	200 ng/mL
Detection Range:	3.13-200 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Tumor immunity;Infection immunity;Developmental science;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Zebrafish HSP-70/HSPA9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish HSP-70/HSPA9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Zebrafish HSP-70/HSPA9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish HSP-70/HSPA9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Environmental Toxicology and Pharmacology

Effects of cyanobacterial oligopeptides: Anabaenopeptin-B, aeruginosin-98B and their mixture on oxidative stress biomarkers, HSP70 production and tyrosine phosphatase activity in three aquatic invertebrate species: Brachionus calyciflorus, Thamnocephalus

日期：2025-08-15 IF：4.2

標準曲線

Concentration (ng/mL)	OD	Corrected OD
200.00	1.946	1.865
100.00	1.686	1.605
50.00	1.157	1.076
25.00	0.813	0.732
12.50	0.617	0.536
6.25	0.326	0.245
3.13	0.226	0.145
0.00	0.081	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant HSP-70/HSPA9 and the recovery rates were calculated by comparing the measured value to the expected amount of HSP-70/HSPA9 in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	87-95%	91%
Heparin plasma(n=5)	82-95%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HSP-70/HSPA9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.