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小鼠血管粘附蛋白1(VAP-1)微量上樣酶聯(lián)免疫吸附檢測(cè)試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK9608MS

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview

Product name:	Mouse VAP-1(VascularAdhesion Protein 1) Microsample ELISA Kit
Reactivity:	Mouse
Alternative Names:	AOC3 ; HPAO; SSAO; Amine oxidase; copper containing 3
Assay Type:	Sandwich
Sensitivity:	0.1 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Immunology;Signal Transduction
Uniprot ID:	O70423
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VAP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VAP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VAP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VAP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	1.961	1.872
5.00	1.590	1.501
2.50	1.261	1.172
1.25	0.884	0.795
0.63	0.538	0.449
0.31	0.351	0.262
0.16	0.216	0.127
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant VAP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of VAP-1 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-90%	87%
EDTA plasma(n=5)	84-121%	103%
Heparin plasma(n=5)	91-111%	101%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VAP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.