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小鼠抗心磷脂抗體IgM(ACA-IgM)酶聯(lián)免疫吸附檢測試劑盒

一鍵復(fù)制產(chǎn)品信息

ELK9760

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下載說明 ①

Overview

Product name:	Mouse ACA-IgM(Anti-Cardiolipin Antibody IgM) ELISA Kit
Reactivity:	Mouse
Alternative Names:	ACA-IgM; Anti-Cardiolipin Antibody IgM
Assay Type:	Sandwich
Sensitivity:	0.086 ng/mL
Standard:	20 ng/mL
Detection Range:	0.31-20 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Cardiovascular biology
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACA-IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACA-IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACA-IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACA-IgM in the samples is then determined by comparing the OD of the samples to the standard curve.

標(biāo)準(zhǔn)曲線

Concentration (ng/mL)	OD	Corrected OD
20.00	2.135	2.041
10.00	1.668	1.574
5.00	1.211	1.117
2.50	0.796	0.702
1.25	0.614	0.520
0.63	0.318	0.224
0.32	0.235	0.141
0.00	0.094	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant ACA-IgM and the recovery rates were calculated by comparing the measured value to the expected amount of ACA-IgM in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	84-98%	91%
Heparin plasma(n=5)	90-105%	97%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACA-IgM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.