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14-3-3 ε rabbit pAb

一鍵復(fù)制產(chǎn)品信息

ES1541

規(guī)格：	價格：
50μL	￥1280.00
100μL	￥1980.00

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Overview

Product name:	14-3-3 ε rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	YWHAE; 14-3-3 protein epsilon; 14-3-3E
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human 14-3-3 epsilon. AA range:206-255
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	29kD
GeneID:	7531
Human Swiss-Prot No:	P62258
Cellular localization:	Nucleus . Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .
Background:	This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008],

Western Blot analysis of various cells using 14-3-3 ε Polyclonal Antibody diluted at 1:1000
Western blot analysis of lysates from HepG2 and Jurkat cells, using 14-3-3 epsilon Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:2

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