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Claudin-1 rabbit pAb

一鍵復(fù)制產(chǎn)品信息

ES1985

規(guī)格：	價(jià)格：
50μL	￥1280.00
100μL	￥1980.00

下載說(shuō)明 ①

Overview

Product name:	Claudin-1 rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	CLDN1; CLD1; SEMP1; Claudin-1; Senescence-associated epithelial membrane protein
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human Claudin 1. AA range:162-211
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	22kD
GeneID:	9076
Human Swiss-Prot No:	O95832
Cellular localization:	Cell junction, tight junction . Cell membrane ; Multi-pass membrane protein . Basolateral cell membrane . Associates with CD81 and the CLDN1-CD81 complex localizes to the basolateral cell membrane. .
Background:	Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008],

Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Immunofluorescence analysis of Hela cell. 1,Claudin-1 Polyclonal Antibody(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:RS3211 was diluted at 1:1000(room temperature, 50min). 3 DAPI(blue) 10min.
Western Blot analysis of various cells using Claudin-1 Polyclonal Antibody diluted at 1:1000
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Claudin 1 Antibody. The picture on the right is blocked with the synthesized peptide.

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