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DGK-ι rabbit pAb

一鍵復制產品信息

ES2166

規(guī)格：	價格：
50μL	￥1280.00
100μL	￥1980.00

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Overview

Product name:	DGK-ι rabbit pAb
Reactivity:	Human;Rat
Alternative Names:	DGKI; Diacylglycerol kinase iota; DAG kinase iota; Diglyceride kinase iota; DGK-iota
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human DGKI. AA range:991-1040
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	160kD
GeneID:	9162
Human Swiss-Prot No:	O75912
Cellular localization:	Cell projection, axon . Cell projection, dendrite . Cell junction, synapse, presynapse . Cell junction, synapse, postsynapse . Cell junction, synapse, postsynaptic density . Cell junction, synapse, synaptic cell membrane . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane . Cytoplasm, cytosol . Nucleus . Excluded from inhibitory synapses (By similarity). Localization between cytoplasm and nucleus is regulated by protein kinase C (PubMed:9830018). Both in the detergent soluble and particulate fractions (By similarity). .
Background:	This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008],

Western Blot analysis of various cells using DGK-ι Polyclonal Antibody diluted at 1:1000
Immunofluorescence analysis of HeLa cells, using DGKI Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293 cells, using DGKI Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human spleen tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200

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