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Dio-1 rabbit pAb

一鍵復制產品信息

ES2172

規(guī)格：	價格：
50μL	￥1280.00
100μL	￥1980.00

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Overview

Product name:	Dio-1 rabbit pAb
Reactivity:	Human;Mouse
Alternative Names:	DIDO1; C20orf158; DATF1; KIAA0333; Death-inducer obliterator 1; DIO-1; hDido1; Death-associated transcription factor 1; DATF-1
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human DIDO1. AA range:161-210
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	244kD
GeneID:	11083
Human Swiss-Prot No:	Q9BTC0
Cellular localization:	Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Translocates to the nucleus after pro-apoptotic stimuli (By similarity). Translocates to the mitotic spindle upon loss of interaction with H3K4me3 during early mitosis. .
Background:	Apoptosis, a major form of cell death, is an efficient mechanism for eliminating unwanted cells and is of central importance for development and homeostasis in metazoan animals. In mice, the death inducer-obliterator-1 gene is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. This gene is similar to the mouse gene and therefore is thought to be involved in apoptosis. Alternatively spliced transcripts have been found for this gene, encoding multiple isoforms. [provided by RefSeq, Jul 2008],

Western Blot analysis of various cells using Dio-1 Polyclonal Antibody diluted at 1:2000
Western blot analysis of lysates from 293 cells, using DIDO1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of the lysates from HepG2 cells using DNL1 antibody.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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