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產(chǎn)品中心

Factor VIII rabbit pAb

一鍵復(fù)制產(chǎn)品信息

ES2314

規(guī)格：	價(jià)格：
50μL	￥1280.00
100μL	￥1980.00

下載說(shuō)明 ①

Overview

Product name:	Factor VIII rabbit pAb
Reactivity:	Human;Mouse
Alternative Names:	F8; F8C; Coagulation factor VIII; Antihemophilic factor; AHF; Procoagulant component
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human Factor VIII. AA range:2161-2210
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	300kD
GeneID:	2157
Human Swiss-Prot No:	P00451
Cellular localization:	Secreted, extracellular space.
Background:	This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008],

Western Blot analysis of various cells using Factor VIII Polyclonal Antibody
Western Blot analysis of HuvEc cells using Factor VIII Polyclonal Antibody
Western blot analysis of lysates from HUVEC cells, using Factor VIII Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).

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