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Overview

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  Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
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  Western blot analysis of lysates from 1) 3T3 , 2) KB ,3) K562 ,4) 293T cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. （Red） Actin β Monoclonal Antibody(5B7) (cat:YM302
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  Western Blot analysis of various cells using FN1 Polyclonal Antibody diluted at 1:2000
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  Western Blot analysis of HeLa cells using FN1 Polyclonal Antibody diluted at 1:2000