Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human MARK1/2/3/4. AA range:181-230
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
89kD
GeneID:
4139/2011/4140/57787
Human Swiss-Prot No:
Q9P0L2/Q7KZI7/P27448/Q96L34
Cellular localization:
Cell membrane ; Peripheral membrane protein . Cytoplasm, cytoskeleton . Cytoplasm . Cell projection, dendrite . Appears to localize to an intracellular network. .
Background:
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation on Thr-215 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39.,function:May play a role in cytoskeletal stability.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. MARK subfamily.,similarity:Contains 1 KA1 (kinase-associated) domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 UBA domain.,subcellular location:Appears to localize to an intracellular network.,tissue specificity:Highly expressed in heart, skeletal muscle, brain, fetal brain and fetal kidney.,