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產(chǎn)品中心

MRP-L50 rabbit pAb

一鍵復(fù)制產(chǎn)品信息

ES2843

規(guī)格：	價格：
50μL	￥1280.00
100μL	￥1980.00

下載說明 ①

Overview

Product name:	MRP-L50 rabbit pAb
Reactivity:	Human;Rat;Mouse;
Alternative Names:	MRPL50; 39S ribosomal protein L50; mitochondrial; L50mt; MRP-L50
Source:	Rabbit
Dilutions:	WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen:	The antiserum was produced against synthesized peptide derived from human MRPL50. AA range:109-158
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	22kD
GeneID:	54534
Human Swiss-Prot No:	Q8N5N7
Cellular localization:	Mitochondrion .
Background:	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q. [provided by RefSeq, Jul 2008],

Western Blot analysis of various cells using MRP-L50 Polyclonal Antibody
Western blot analysis of lysates from COLO and HT-29 cells, using MRPL50 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).

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