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  Western blot analysis of KB MCF-7 using p-IRS-1 (Y896) antibody. Antibody was diluted at 1:500
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  Western blot analysis of lysates from HepG2 cells treated with Na3VO4 0.3mM 40', using IRS-1 (Phospho-Tyr896) Antibody. The lane on the right is blocked with the phospho peptide.
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  Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).